Primers design for the detection and genotyping of the Canine Distemper Virus

Canine Distemper Virus (CDV) has potential hosts, all carnivores, including wild animals. The associated disease, called canine distemper (CD) a high prevalence and is highly contagious, being one of the main causes death in domestic canids. Although it monotypic virus, that is, with little genetic preference, H gene variation, which would produce different strains some more virulent than others tropism. It because this specific specification patients still get sick, despite vaccinated, since eightfold vaccine protects against strain virus: America-1. Thus, any carnivore susceptible to virus confronted not been vaccinated as likely become ill an animal simply never viral disease. To face situation, essential know characteristics genotypes present country. Through previous studies, known Chile there are at least two fourteen VDC lineages, but does imply they only ones country, why proposed propose prior Polymerase Chain Reaction protocol. Reverse transcription using both matches for N (lineages: America-1 European) shed light on genotype found samples, may even belong either lineages described above For generation RT-PCR protocol capable detecting VDC, use Genbank® genomic database conjunction silico design primers very interesting, indicating whether corresponds American-1 or European lineage.